Tuberculosis-infectious disease caused by Mycobacterium Tuberculosis- attacks the lungsCan be acquired through inhalationPathogen of the mammalian respiratory system (infects lungs)Neither gram-positive nor gram-negative (acid-fast staining used)Cell wall highly concentrated with lipids-unique propertyDivides slower than other bacteriaTaken up by alveolar macrophages-doesn’t get digested; thus multipliesSymptoms: cough, fever, loss of appetite (infection can spread to other parts of the body)PSTP phosphorylates proteins in mycobacterium tuberculosis, which help them sustain and multiply.
ORGANISM: MYCOBACTERIUM TUBERCULOSIS H37Rv
EC# 3.1.3.16
TARGET ENZYME: SERINE THREONINE PROTEIN PHOSPHATASE PSTP
NCBI GENE # OR REFSEQ #:887070
BACKGROUND INFORMATION:
Tuberculosis-infectious disease caused by Mycobacterium Tuberculosis- attacks the lungsCan be acquired through inhalationPathogen of the mammalian respiratory system (infects lungs)Neither gram-positive nor gram-negative (acid-fast staining used)Cell wall highly concentrated with lipids-unique propertyDivides slower than other bacteriaTaken up by alveolar macrophages-doesn’t get digested; thus multipliesSymptoms: cough, fever, loss of appetite (infection can spread to other parts of the body)PSTP phosphorylates proteins in mycobacterium tuberculosis, which help them sustain and multiply.IMAGE:
CDS GENE SEQUENCE:
>gi|57116681:c23181-21637 Mycobacterium tuberculosis H37Rv, complete genome
GTGGCGCGCGTGACCCTGGTCCTGCGATACGCGGCGCGCAGCGATCGCGGCTTGGTACGCGCCAACAACGAAGACTCGG
TCTACGCTGGGGCACGGCTATTGGCCCTGGCCGACGGCATGGGTGGGCATGCGGCCGGCGAGGTGGCGTCCCAGTTGG
TGATTGCCGCATTGGCCCATCTCGATGACGACGAGCCCGGTGGCGATCTGCTGGCCAAGCTGGATGCCGCGGTGCGCGC
CGGCAACTCGGCTATCGCAGCGCAAGTCGAGATGGAGCCCGATCTCGAAGGCATGGGTACCACGCTCACCGCAATCCTGT
TCGCGGGCAACCGGCTCGGCCTGGTGCATATCGGTGACTCGCGCGGTTACCTGCTGCGCGACGGTGAGCTGACGCAGAT
CACCAAGGACGACACGTTTGTCCAAACGCTGGTCGACGAAGGCCGGATCACCCCGGAGGAGGCGCACAGCCACCCGCAAC
GCTCGTTGATCATGCGGGCGTTGACCGGCCATGAGGTCGAACCGACGCTGACCATGCGAGAAGCCCGCGCCGGTGATCG
TTACCTGCTGTGCTCGGACGGGTTGTCCGATCCGGTTAGCGATGAAACTATCCTCGAGGCCCTGCAGATCCCCGAGGTTG
CCGAGAGCGCTCACCGCCTCATTGAACTGGCGCTGCGCGGCGGCGGCCCCGACAACGTCACTGTCGTCGTCGCCGACGT
CGTCGACTACGACTACGGCCAGACCCAACCGATTCTGGCCGGGGCGGTCTCAGGCGACGACGACCAACTGACCCTGCCCA
ACACCGCCGCCGGCCGGGCCTCTGCCATCAGCCAGCGCAAGGAGATCGTTAAACGCGTTCCGCCACAGGCCGATACATTC
AGTCGGCCACGGTGGTCGGGCCGACGGCTAGCATTCGTTGTCGCACTGGTGACCGTGCTGATGACTGCGGGCCTGCTCA
TTGGTCGCGCGATCATCCGCAGCAACTACTACGTAGCGGACTACGCCGGCAGCGTGTCCATCATGCGGGGGATTCAAGGG
TCGCTACTGGGCATGTCCCTGCACCAGCCTTACCTGATGGGCTGCCTCAGCCCGCGTAACGAGCTGTCGCAGATCAGCTAC
GGACAGTCTGGGGGCCCTCTCGACTGCCATCTGATGAAACTGGAGGATCTGCGACCGCCGGAGCGCGCACAGGTTCGGGC
CGGTCTCCCGGCCGGCACTCTCGATGACGCCATCGGGCAGTTGCGCGAACTGGCGGCCAACTCCCTGCTGCCGCCTTGCC
CGGCGCCGCGTGCCACGTCCCCGCCCGGGCGCCCGGCCCCACCCACCACCAGCGAGACAACCGAACCAAACGTCACCTCC
TCGCCAGCCTCTCCATCACCCACCACCTCCGCGCCGGCCCCCACCGGAACTACTCCTGCCATCCCCACGAGTGCCTCCCCG
GCAGCGCCCGCGTCGCCGCCGACGCCTTGGCCCGTCACCAGCTCGCCGACGATGGCCGCACTTCCGCCACCCCCGCCTCA
GCCGGGCATCGACTGCCGGGCGGCGGCATGA
AMINO ACID SEQUENCE:
>1TXO:A|PDBID|CHAIN|SEQUENCE
MARVTLVLRYAARSDRGLVRANNEDSVYAGARLLALADGMGGHAAGEVASQLVIAALAHLDDDEPGGDLLAKLDAA
VRAGNSAIAAQVEMEPDLEGMGTTLTAILFAGNRLGLVHIGDSRGYLLRDGELTQITKDDTFVQTLVDEGRITPEEAHSH
PQRSLIMRALTGHEVEPTLTMREARAGDRYLLCSDGLSDPVSDETILEALQIPEVAESAHRLIELALRGGGPDNVTVVVADVVD
serine/threonine phosphatase [Mycobacterium tuberculosis H37Rv]
NCBI Reference Sequence: NP_214532.1GenPept Graphics
>gi|15607160|ref|NP_214532.1| serine/threonine phosphatase [Mycobacterium tuberculosis H37Rv]
MARVTLVLRYAARSDRGLVRANNEDSVYAGARLLALADGMGGHAAGEVASQLVIAALAHLDDDEPGGDLL AKLDAAVRAGNSAIAAQVEMEPDLEGMGTTLTAILFAGNRLGLVHIGDSRGYLLRDGELTQITKDDTFVQ TLVDEGRITPEEAHSHPQRSLIMRALTGHEVEPTLTMREARAGDRYLLCSDGLSDPVSDETILEALQIPE VAESAHRLIELALRGGGPDNVTVVVADVVDYDYGQTQPILAGAVSGDDDQLTLPNTAAGRASAISQRKEI VKRVPPQADTFSRPRWSGRRLAFVVALVTVLMTAGLLIGRAIIRSNYYVADYAGSVSIMRGIQGSLLGMS LHQPYLMGCLSPRNELSQISYGQSGGPLDCHLMKLEDLRPPERAQVRAGLPAGTLDDAIGQLRELAANSL LPPCPAPRATSPPGRPAPPTTSETTEPNVTSSPASPSPTTSAPAPTGTTPAIPTSASPAAPASPPTPWPV TSSPTMAALPPPPPQPGIDCRAAA
Molecular Weight - Dr. B
53811.9
Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient- Dr. B
27640
Filtered DNA Sequence:
ATGGCACGCGTTACCCTCGTTCTGCGTTACGCTGCGCGTTCTGACCGTGGTCTGGTTCGC
GCAAACAATGAGGACTCTGTTTACGCGGGTGCTCGTCTCCTCGCGCTGGCCGACGGCATG
GGTGGTCACGCTGCCGGTGAAGTTGCGTCTCAGCTGGTTATCGCAGCCCTGGCGCATCTC
GACGATGACGAACCGGGTGGTGACCTCCTGGCGAAACTGGACGCCGCAGTGCGTGCCGGT
AACTCTGCGATTGCGGCGCAGGTTGAAATGGAACCGGACCTGGAGGGTATGGGTACCACC
CTGACGGCGATCCTGTTCGCGGGTAATCGTCTGGGTCTCGTTCACATCGGTGACTCTCGT
GGTTACCTGCTGCGTGACGGTGAACTGACTCAGATCACCAAAGACGACACCTTTGTTCAG
ACTCTGGTTGACGAAGGTCGTATCACCCCGGAAGAAGCGCACTCTCATCCGCAACGTTCT
CTGATCATGCGCGCTCTCACGGGCCACGAAGTTGAACCAACCCTCACCATGCGTGAAGCG
CGTGCGGGTGACCGCTACCTCCTCTGCTCTGATGGTCTCTCTGACCCTGTTTCTGACGAA
ACCATCCTCGAAGCGCTGCAGATTCCGGAGGTTGCTGAATCCGCCCATCGCCTGATTGAA
CTGGCGCTCCGTGGTGGCGGTCCGGATAATGTTACTGTTGTAGTTGCCGATGTCGTTGAC
TAA
PDB STRUCTURE IDENTIFIER: 2CM1
Molecular Weight of protein: 60335.4Forward Primer:
TACTTCCAATCCATGGCACGCGTTACCCTCGT
32 bp
GC Content: 53.1%
0mM Mg: 67.1C
1.5mM Mg: 73.8C
2mM Mg: 74.3C
4mM Mg: 75.1C
6mM Mg: 75.5C
Reverse Primer:
TATCCACCTTTACTGTTAGTCAACGACATCGGCAA
35 bp
GC Content: 42.9%
0mM Mg: 63.3C
1.5mM Mg: 70.8C
2mM Mg: 71.3C
4mM Mg: 72.2C
6mM Mg: 72.7C
VirtualPlasmid
TAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTTCCAATCCATGGCACGCGTTACCCTCGTTCTGCGTTACGCTGCGCGTTCTGACCGTGGTCTGGTTCGCGCAAACAATGAGGACTCTGTTTACGCGGGTGCTCGTCTCCTCGCGCTGGCCGACGGCATGGGTGGTCACGCTGCCGGTGAAGTTGCGTCTCAGCTGGTTATCGCAGCCCTGGCGCATCTCGACGATGACGAACCGGGTGGTGACCTCCTGGCGAAACTGGACGCCGCAGTGCGTGCCGGTAACTCTGCGATTGCGGCGCAGGTTGAAATGGAACCGGACCTGGAGGGTATGGGTACCACCCTGACGGCGATCCTGTTCGCGGGTAATCGTCTGGGTCTCGTTCACATCGGTGACTCTCGTGGTTACCTGCTGCGTGACGGTGAACTGACTCAGATCACCAAAGACGACACCTTTGTTCAGACTCTGGTTGACGAAGGTCGTATCACCCCGGAAGAAGCGCACTCTCATCCGCAACGTTCTCTGATCATGCGCGCTCTCACGGGCCACGAAGTTGAACCAACCCTCACCATGCGTGAAGCGCGTGCGGGTGACCGCTACCTCCTCTGCTCTGATGGTCTCTCTGACCCTGTTTCTGACGAAACCATCCTCGAAGCGCTGCAGATTCCGGAGGTTGCTGAATCCGCCCATCGCCTGATTGAACTGGCGCTCCGTGGTGGCGGTCCGGATAATGTTACTGTTGTAGTTGCCGATGTCGTTGACTAACAGTAAAGGTGGATACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGCACCACCACCACCACCACTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGATTGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAATTAATTCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGTTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTAGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCTGCGGTAAAGCTCATCAGCGTGGTCGTGAAGCGATTCACAGATGTCTGCCTGTTCATCCGCGTCCAGCTCGTTGAGTTTCTCCAGAAGCGTTAATGTCTGGCTTCTGATAAAGCGGGCCATGTTAAGGGCGGTTTTTTCCTGTTTGGTCACTGATGCCTCCGTGTAAGGGGGATTTCTGTTCATGGGGGTAATGATACCGATGAAACGAGAGAGGATGCTCACGATACGGGTTACTGATGATGAACATGCCCGGTTACTGGAACGTTGTGAGGGTAAACAACTGGCGGTATGGATGCGGCGGGACCAGAGAAAAATCACTCAGGGTCAATGCCAGCGCTTCGTTAATACAGATGTAGGTGTTCCACAGGGTAGCCAGCAGCATCCTGCGATGCAGATCCGGAACATAATGGTGCAGGGCGCTGACTTCCGCGTTTCCAGACTTTACGAAACACGGAAACCGAAGACCATTCATGTTGTTGCTCAGGTCGCAGACGTTTTGCAGCAGCAGTCGCTTCACGTTCGCTCGCGTATCGGTGATTCATTCTGCTAACCAGTAAGGCAACCCCGCCAGCCTAGCCGGGTCCTCAACGACAGGAGCACGATCATGCGCACCCGTGGGGCCGCCATGCCGGCGATAATGGCCTGCTTCTCGCCGAAACGTTTGGTGGCGGGACCAGTGACGAAGGCTTGAGCGAGGGCGTGCAAGATTCCGAATACCGCAAGCGACAGGCCGATCATCGTCGCGCTCCAGCGAAAGCGGTCCTCGCCGAAAATGACCCAGAGCGCTGCCGGCACCTGTCCTACGAGTTGCATGATAAAGAAGACAGTCATAAGTGCGGCGACGATAGTCATGCCCCGCGCCCACCGGAAGGAGCTGACTGGGTTGAAGGCTCTCAAGGGCATCGGTCGAGATCCCGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCCAGCCAGACGCAGACGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCGCGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAGTCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCCGGGATCTCGACGCTCTCCCTTATGCGACTCCTGCATTAGGAAGCAGCCCAGTAGTAGGTTGAGGCCGTTGAGCACCGCCGCCGCAAGGAATGGTGCATGCAAGGAGATGGCGCCCAACAGTCCCCCGGCCACGGGGCCTGCCACCATACCCACGCCGAAACAAGCGCTCATGAGCCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGGCGCCAGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCTCGATCCCGCGAAAT